Journal: Frontiers in Neuroscience

Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle

doi: 10.3389/fnins.2023.1247375

Figure Lengend Snippet: Apposition of aPC neuron axonal boutons to postsynaptic molecules. (A) Apposition of mCherry (red)/VGLUT1 (green) coexpressing aPC neuron axonal boutons (arrows) to Homer 1b/c puncta (blue) (arrowheads). Photographs of amOT layer Ib for the control and attraction-learned mice (left panels) and of lOT layer III for the control and aversion-learned mice (right panels) are indicated. Scale bar, 1 μm. (B) Percentage of apposition of axonal boutons to Homer 1b/c puncta in the amOT for the control and attraction-learned mice (left panel) as well as in the lOT for the control and aversion-learned mice (right panel). Average ± SD; n = 4 in each column, from rostrocaudally distinct 2 areas of layers Ib, II and III of 2 mice. *, p < 0.05; **, p < 0.01 ( t -test).

Article Snippet: The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Learning-dependent structural plasticity of intracortical and sensory connections to functional domains of the olfactory tubercle

doi: 10.3389/fnins.2023.1247375

Figure Lengend Snippet: Apposition of OB neuron axonal boutons to postsynaptic molecules. (A) Apposition of mCherry (red)/VGLUT1 (green) coexpressing OB neuron axonal boutons (arrows) to Homer 1b/c puncta (blue) (arrowheads). Photographs in amOT layer Ia for the control and attraction-learned mice (left panels) and in lOT layer Ia for the control and aversion-learned mice (right panels) are indicated. Scale bar, 1 μm. (B) Percentages of apposition of axonal boutons to Homer 1b/c puncta in the amOT for the control and attraction-learned mice (left panel) as well as in the lOT for the control and aversion-learned mice (right panel). Average ± SD; n = 4 in each column, from rostrocaudally distinct 2 areas of layer Ia of 2 mice. *, p < 0.05; **, p < 0.01 ( t -test).

Article Snippet: The primary antibodies were mouse anti-Cre recombinase monoclonal antibody (1:200, MAB3120; Merck Millipore, Darmstadt, Germany), rabbit anti-c-fos polyclonal antibody (1:500; ab-190,289; Abcam, Cambridge, United Kingdom), mouse anti-mCherry monoclonal antibody (1:500; #632543; Takara Bio, Shiga, Japan), guinea pig anti-VGLUT1 polyclonal antibody (1:500; AB5905; Merck Millipore, Burlington, MA, United States), and rabbit anti-Homer 1b/c antibody (1:200; #160023; Synaptic Systems, Goettingen, Germany).

Techniques:

Journal: Cell Reports

Article Title: Axonal plasticity in response to active forces generated through magnetic nano-pulling

doi: 10.1016/j.celrep.2022.111912

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Homer 1b/c , Synaptic Systems , #160 023 RRID: AB_2619858.

Techniques: Recombinant, Produced, Reverse Transcription, Software, Electron Microscopy